Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4311738..4312235 Replicon chromosome
Accession NZ_CP116987
Organism Escherichia coli strain MLI107

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL408_RS20995 Protein ID WP_001173343.1
Coordinates 4311738..4311911 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL408_RS21000 Protein ID WP_000398619.1
Coordinates 4311939..4312235 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL408_RS20985 (4308408) 4308408..4309865 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
NL408_RS20990 (4310102) 4310102..4311619 + 1518 WP_001295074.1 lysine--tRNA ligase -
NL408_RS20995 (4311738) 4311738..4311911 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL408_RS21000 (4311939) 4311939..4312235 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL408_RS21005 (4312462) 4312462..4312734 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NL408_RS21010 (4312746) 4312746..4312976 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
NL408_RS21015 (4313157) 4313157..4314788 + 1632 WP_001216486.1 sensor histidine kinase -
NL408_RS21020 (4314785) 4314785..4315504 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269664 WP_001173343.1 NZ_CP116987:c4311911-4311738 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269664 WP_000398619.1 NZ_CP116987:c4312235-4311939 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References