Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3551911..3552748 Replicon chromosome
Accession NZ_CP116987
Organism Escherichia coli strain MLI107

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NL408_RS17455 Protein ID WP_000227784.1
Coordinates 3552206..3552748 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NL408_RS17450 Protein ID WP_001297137.1
Coordinates 3551911..3552222 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL408_RS17425 (3546931) 3546931..3547878 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
NL408_RS17430 (3547900) 3547900..3549891 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NL408_RS17435 (3549881) 3549881..3550495 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NL408_RS17440 (3550495) 3550495..3550824 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NL408_RS17445 (3550836) 3550836..3551726 + 891 WP_000971336.1 heme o synthase -
NL408_RS17450 (3551911) 3551911..3552222 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NL408_RS17455 (3552206) 3552206..3552748 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NL408_RS17460 (3552804) 3552804..3553739 - 936 WP_001297127.1 tetratricopeptide repeat protein -
NL408_RS17465 (3554147) 3554147..3555511 + 1365 WP_001000975.1 MFS transporter -
NL408_RS17470 (3555639) 3555639..3556130 - 492 WP_001138904.1 nucleotide binding protein YajQ -
NL408_RS17475 (3556298) 3556298..3557209 + 912 WP_000705877.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T269659 WP_000227784.1 NZ_CP116987:3552206-3552748 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT269659 WP_001297137.1 NZ_CP116987:3551911-3552222 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References