Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 304706..305506 Replicon chromosome
Accession NZ_CP116987
Organism Escherichia coli strain MLI107

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NL408_RS01405 Protein ID WP_000342449.1
Coordinates 304979..305506 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NL408_RS01400 Protein ID WP_001277108.1
Coordinates 304706..304972 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL408_RS01380 (300364) 300364..301032 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NL408_RS01385 (301025) 301025..302083 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NL408_RS01390 (302328) 302328..303182 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NL408_RS01395 (303453) 303453..304556 + 1104 WP_255141743.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NL408_RS01400 (304706) 304706..304972 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NL408_RS01405 (304979) 304979..305506 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NL408_RS01410 (305503) 305503..305886 - 384 WP_206311438.1 aspartate 1-decarboxylase autocleavage activator PanM -
NL408_RS01415 (306310) 306310..307419 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NL408_RS01420 (307467) 307467..308393 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NL408_RS01425 (308390) 308390..309667 + 1278 WP_000803784.1 branched chain amino acid ABC transporter permease LivM -
NL408_RS01430 (309664) 309664..310431 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T269647 WP_000342449.1 NZ_CP116987:304979-305506 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT269647 WP_001277108.1 NZ_CP116987:304706-304972 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References