Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 64046..64849 Replicon plasmid pE6474.1
Accession NZ_CP116982
Organism Escherichia coli strain E6474

Toxin (Protein)


Gene name KacT Uniprot ID G3CAI8
Locus tag PO910_RS23370 Protein ID WP_000348883.1
Coordinates 64046..64576 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag PO910_RS23375 Protein ID WP_001275013.1
Coordinates 64580..64849 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO910_RS23340 (59714) 59714..60490 + 777 WP_039052454.1 hypothetical protein -
PO910_RS23345 (60492) 60492..62756 + 2265 WP_015060475.1 UvrD-helicase domain-containing protein -
PO910_RS23350 (63174) 63174..63245 - 72 Protein_59 hypothetical protein -
PO910_RS23355 (63229) 63229..63459 + 231 WP_001261274.1 type II toxin-antitoxin system VapB family antitoxin -
PO910_RS23360 (63456) 63456..63772 + 317 Protein_61 type II toxin-antitoxin system VapC family toxin -
PO910_RS23365 (63828) 63828..64045 - 218 Protein_62 transposase -
PO910_RS23370 (64046) 64046..64576 - 531 WP_000348883.1 GNAT family N-acetyltransferase Toxin
PO910_RS23375 (64580) 64580..64849 - 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
PO910_RS23380 (65104) 65104..65801 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
PO910_RS23385 (66075) 66075..66618 + 544 Protein_66 fimbrial protein -
PO910_RS23390 (66671) 66671..67363 + 693 WP_001223973.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / sul3 - 1..156174 156174


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20321.20 Da        Isoelectric Point: 6.4889

>T269638 WP_000348883.1 NZ_CP116982:c64576-64046 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT269638 WP_001275013.1 NZ_CP116982:c64849-64580 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAI8

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References