269616

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1052423..1053260 Replicon chromosome
Accession NZ_CP116981
Organism Escherichia coli strain E6474

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag PO910_RS05065 Protein ID WP_000227784.1
Coordinates 1052423..1052965 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag PO910_RS05070 Protein ID WP_001297137.1
Coordinates 1052949..1053260 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO910_RS05045 (1047962) 1047962..1048873 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
PO910_RS05050 (1049041) 1049041..1049532 + 492 WP_001138904.1 nucleotide binding protein YajQ -
PO910_RS05055 (1049660) 1049660..1051024 - 1365 WP_001000974.1 MFS transporter -
PO910_RS05060 (1051432) 1051432..1052367 + 936 WP_001365761.1 tetratricopeptide repeat protein -
PO910_RS05065 (1052423) 1052423..1052965 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
PO910_RS05070 (1052949) 1052949..1053260 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
PO910_RS05075 (1053445) 1053445..1054335 - 891 WP_000971336.1 heme o synthase -
PO910_RS05080 (1054347) 1054347..1054676 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
PO910_RS05085 (1054676) 1054676..1055290 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
PO910_RS05090 (1055280) 1055280..1057271 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
PO910_RS05095 (1057293) 1057293..1058240 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T269616 WP_000227784.1 NZ_CP116981:c1052965-1052423 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT269616 WP_001297137.1 NZ_CP116981:c1053260-1052949 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References