Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2573439..2573936 Replicon chromosome
Accession NZ_CP116976
Organism Escherichia coli strain CUVM53

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PQP10_RS12520 Protein ID WP_001173343.1
Coordinates 2573439..2573612 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PQP10_RS12525 Protein ID WP_000398619.1
Coordinates 2573640..2573936 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQP10_RS12510 (2570109) 2570109..2571566 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
PQP10_RS12515 (2571803) 2571803..2573320 + 1518 WP_001295087.1 lysine--tRNA ligase -
PQP10_RS12520 (2573439) 2573439..2573612 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PQP10_RS12525 (2573640) 2573640..2573936 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PQP10_RS12530 (2574163) 2574163..2574435 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PQP10_RS12535 (2574447) 2574447..2574677 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PQP10_RS12540 (2574858) 2574858..2576489 + 1632 WP_001216477.1 sensor histidine kinase -
PQP10_RS12545 (2576486) 2576486..2577205 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269596 WP_001173343.1 NZ_CP116976:c2573612-2573439 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269596 WP_000398619.1 NZ_CP116976:c2573936-2573640 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References