Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 152585..153110 Replicon plasmid pCUVM3.1
Accession NZ_CP116971
Organism Escherichia coli strain CUVM3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag PQP09_RS24910 Protein ID WP_001159871.1
Coordinates 152585..152890 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag PQP09_RS24915 Protein ID WP_000813639.1
Coordinates 152892..153110 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQP09_RS24885 (147587) 147587..148558 - 972 WP_032248840.1 ParB/RepB/Spo0J family plasmid partition protein -
PQP09_RS24890 (148558) 148558..149724 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
PQP09_RS24895 (150312) 150312..150485 - 174 Protein_167 RepB family plasmid replication initiator protein -
PQP09_RS24905 (151920) 151920..152584 - 665 Protein_169 site-specific integrase -
PQP09_RS24910 (152585) 152585..152890 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
PQP09_RS24915 (152892) 152892..153110 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PQP09_RS24920 (153927) 153927..154217 - 291 WP_032248853.1 hypothetical protein -
PQP09_RS24925 (154214) 154214..155341 - 1128 WP_032248854.1 DUF4238 domain-containing protein -
PQP09_RS24930 (155375) 155375..156898 - 1524 WP_227464009.1 hypothetical protein -
PQP09_RS24935 (157175) 157175..157651 - 477 WP_032248785.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..164599 164599


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T269578 WP_001159871.1 NZ_CP116971:c152890-152585 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT269578 WP_000813639.1 NZ_CP116971:c153110-152892 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References