Detailed information of TA system TA026956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 285571..286478
• Replicon: plasmid pEA320
• Accession: NC_017533
• Organism: Pantoea ananatis AJ13355

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: PAJ_RS22205
• Protein ID: WP_019105827.1
• Coordinates: 286011..286478 (+)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: PAJ_RS22200
• Protein ID: WP_014598335.1
• Coordinates: 285571..286014 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAJ_RS22170	280695..281081	-	387	WP_013027868.1	pyrimidine utilization protein C	-
PAJ_RS22175	281107..281859	-	753	WP_014604633.1	pyrimidine utilization protein B	-
PAJ_RS22180	281856..282947	-	1092	WP_013027866.1	pyrimidine utilization protein A	-
PAJ_RS22185	283346..284035	+	690	WP_014598333.1	HTH-type transcriptional regulator RutR	-
PAJ_RS22190	284111..284806	-	696	WP_014598334.1	hypothetical protein	-
PAJ_RS22195	284960..285184	-	225	WP_013027863.1	KTSC domain-containing protein	-
PAJ_RS22200	285571..286014	+	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
PAJ_RS22205	286011..286478	+	468	WP_019105827.1	RES family NAD+ phosphorylase	Toxin
PAJ_RS22210	287123..288517	+	1395	WP_014598337.1	flagellar filament capping protein FliD	-
PAJ_RS22215	289202..290137	+	936	WP_013027859.1	hypothetical protein	-
PAJ_RS22220	290199..290771	-	573	WP_014598338.1	NAD(P)H-dependent oxidoreductase	-
PAJ_RS22835	290859..291092	+	234	WP_013027857.1	DUF333 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vipA/tssB / vipB/tssC / vasE/tssK / dotU/tssL / hcp/tssD / clpV/tssH / vgrG/tssI / icmF/tssM / tssF / tssG / fliC / fliC	1..321744	321744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17121.61 Da
• Isoelectric Point: 5.7185

>T26956 WP_019105827.1 NC_017533:286011-286478 [Pantoea ananatis AJ13355]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPGDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFSAIVRQAVNIPFLFDARFSRQA

Nucleotide

• Length: 468 bp

>T26956 NC_017533:286011-286478 [Pantoea ananatis AJ13355]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTGGGGTCGGCCTGGAGCGGTTTTGGTGCCCGTGAAGGCGGCGGACG
CTGGAACAGCGTCGGCGTATCCATGGTCTACGCTTCTGAAACCGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTGCTGGACTTTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTTCCTGGCGACTGGGCCGCACCGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGGGG
ATCGGTCGCACTTCGCGTGCCCAGTGCCCTTTCGCCCGTGGAATCGAATTATCTTCTCAATCCCGAGCATCCTGAATTCA
GTGCGATCGTCAGACAGGCGGTAAACATCCCCTTCCTTTTTGACGCACGTTTTTCCCGACAGGCATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT26956 WP_014598335.1 NC_017533:285571-286014 [Pantoea ananatis AJ13355]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT26956 NC_017533:285571-286014 [Pantoea ananatis AJ13355]
ATGAAACAGTTCACTTTACCCGTATCCGCTGCAGAACCGGATCGTCTATGGCAATTTGCTGGCCTGAGCAGTGCTGACGG
CGTGATGCTGATGGATCAAATCGATGATGGTCTGGACGGCACGGTAGCCAGTCGCATTGTCACCTGGGCGCAAATCTCTC
AGGCAGAACTGCGCCGTATGACCGGCATTGCGGGCTCAACCTTCACCCGCGGCGTTAAAAGCCGCTTTACTGCGGAGCAG
AGCGAACGGCTGGTGCGTTTTATTCGCGTGATGGATCGTGCGGTTGAGCTGTTTGAGGGCGATAAAGAAAAGGCACGGCA
GTGGCTCAATGAGCCTGCGCGCGCCTTAGGTAATAAAAAACCGGCCGATCTGGTTGCGTCAGAAACCGGGGCTTATGAAG
TGCTGAAACTCATTACCCGGCTTGAACACGGTGTGTATTCTTGA