Detailed information of TA system TA026955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1961189..1961779
• Replicon: chromosome
• Accession: NC_017531
• Organism: Pantoea ananatis AJ13355

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: PAJ_RS09030
• Protein ID: WP_015700048.1
• Coordinates: 1961189..1961521 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: PAJ_RS09035
• Protein ID: WP_014605554.1
• Coordinates: 1961522..1961779 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAJ_RS09005	1957668..1957922	-	255	WP_013026202.1	Arc family DNA-binding protein	-
PAJ_RS09010	1957996..1958700	-	705	WP_014593959.1	phosphohydrolase	-
PAJ_RS09015	1959312..1959989	+	678	WP_013026204.1	hypothetical protein	-
PAJ_RS09020	1960095..1960928	+	834	WP_013026205.1	DgsA anti-repressor MtfA	-
PAJ_RS09030	1961189..1961521	-	333	WP_015700048.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PAJ_RS09035	1961522..1961779	-	258	WP_014605554.1	antitoxin	Antitoxin
PAJ_RS22530	1962127..1962332	-	206	Protein_1782	helix-turn-helix domain-containing protein	-
PAJ_RS09040	1962832..1963794	-	963	WP_041930826.1	ferrochelatase	-
PAJ_RS09045	1964542..1965780	+	1239	WP_041930827.1	MFS transporter	-
PAJ_RS09050	1966056..1966493	-	438	WP_041930828.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (4-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11945.66 Da
• Isoelectric Point: 6.9807

>T26955 WP_015700048.1 NC_017531:c1961521-1961189 [Pantoea ananatis AJ13355]
MERGEIWLVSLDPNAGHEQSGRRPVLIVSPASFNQFTRLPVVVPVTNGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMEARNGKYLERIPEVIVNEVVARLEAILS

Nucleotide

• Length: 333 bp

>T26955 NC_017531:c1961521-1961189 [Pantoea ananatis AJ13355]
ATGGAAAGAGGGGAAATCTGGCTCGTTTCACTAGATCCGAATGCCGGGCATGAACAAAGCGGAAGGCGTCCAGTACTGAT
TGTTTCTCCGGCGTCCTTTAATCAGTTTACGCGGCTACCTGTGGTTGTTCCCGTGACCAACGGCGGAAACTTTGCCCGCG
CAGCGGGGTTTACTGTTTCACTGGATGGCGCAGGAACGAAAACAACGGGGGTAATTCGTTGTGACCAGCCCAGAACCATT
GATATGGAAGCCCGGAACGGCAAATATCTTGAACGAATACCTGAAGTTATTGTGAATGAAGTGGTGGCCAGACTGGAGGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9253.73 Da
• Isoelectric Point: 4.5821

>AT26955 WP_014605554.1 NC_017531:c1961779-1961522 [Pantoea ananatis AJ13355]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDSGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKELL

Nucleotide

• Length: 258 bp

>AT26955 NC_017531:c1961779-1961522 [Pantoea ananatis AJ13355]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCTGTCATGCTGGCGGTCCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAGTGGCTGCCTCATAATTGAACCTCAAAAACGTCCCCGCTATTCAC
TCGAAGAACTACTGGCGCAGTGCGATCCGCACGCTGAAATTAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGCTCCTGTAG