Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4466694..4467191 Replicon chromosome
Accession NZ_CP116931
Organism Escherichia coli strain MLI106K1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL422_RS22135 Protein ID WP_001173343.1
Coordinates 4466694..4466867 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL422_RS22140 Protein ID WP_000398619.1
Coordinates 4466895..4467191 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL422_RS22125 (4463364) 4463364..4464821 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
NL422_RS22130 (4465058) 4465058..4466575 + 1518 WP_001550472.1 lysine--tRNA ligase -
NL422_RS22135 (4466694) 4466694..4466867 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL422_RS22140 (4466895) 4466895..4467191 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL422_RS22145 (4467420) 4467420..4467692 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NL422_RS22150 (4467704) 4467704..4467934 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NL422_RS22155 (4468114) 4468114..4469745 + 1632 WP_001216477.1 sensor histidine kinase -
NL422_RS22160 (4469742) 4469742..4470461 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269512 WP_001173343.1 NZ_CP116931:c4466867-4466694 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269512 WP_000398619.1 NZ_CP116931:c4467191-4466895 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References