Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3859828..3860404 Replicon chromosome
Accession NZ_CP116929
Organism Escherichia coli strain MLI105

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NL421_RS19125 Protein ID WP_001297643.1
Coordinates 3860117..3860404 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag NL421_RS19120 Protein ID WP_000063160.1
Coordinates 3859828..3860130 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL421_RS19105 (3856424) 3856424..3858574 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
NL421_RS19110 (3858624) 3858624..3858827 + 204 WP_000467859.1 YbdD/YjiX family protein -
NL421_RS19115 (3858838) 3858838..3859794 + 957 WP_001297640.1 GTPase -
NL421_RS19120 (3859828) 3859828..3860130 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
NL421_RS19125 (3860117) 3860117..3860404 - 288 WP_001297643.1 BrnT family toxin Toxin
NL421_RS19130 (3860603) 3860603..3861299 - 697 Protein_3705 IS1 family transposase -
NL421_RS19135 (3861614) 3861614..3862846 + 1233 WP_001825915.1 multidrug efflux MFS transporter MdtM -
NL421_RS19140 (3862887) 3862887..3864167 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3860603..3860812 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T269489 WP_001297643.1 NZ_CP116929:c3860404-3860117 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT269489 WP_000063160.1 NZ_CP116929:c3860130-3859828 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References