Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4137617..4138193 Replicon chromosome
Accession NZ_CP116925
Organism Escherichia coli strain MLI104K2

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag NL420_RS21135 Protein ID WP_001360091.1
Coordinates 4137906..4138193 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NL420_RS21130 Protein ID WP_000063156.1
Coordinates 4137617..4137919 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL420_RS21115 (4134213) 4134213..4136363 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NL420_RS21120 (4136413) 4136413..4136616 + 204 WP_000467859.1 YbdD/YjiX family protein -
NL420_RS21125 (4136627) 4136627..4137583 + 957 WP_001297640.1 GTPase -
NL420_RS21130 (4137617) 4137617..4137919 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NL420_RS21135 (4137906) 4137906..4138193 - 288 WP_001360091.1 BrnT family toxin Toxin
NL420_RS21140 (4138392) 4138392..4139089 - 698 Protein_4034 IS1 family transposase -
NL420_RS21145 (4139398) 4139398..4140630 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
NL420_RS21150 (4140671) 4140671..4141951 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T269470 WP_001360091.1 NZ_CP116925:c4138193-4137906 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT269470 WP_000063156.1 NZ_CP116925:c4137919-4137617 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References