269446

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4032971..4033547 Replicon chromosome
Accession NZ_CP116923
Organism Escherichia coli strain MLI104K1

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag NL419_RS20520 Protein ID WP_001360091.1
Coordinates 4033260..4033547 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NL419_RS20515 Protein ID WP_000063156.1
Coordinates 4032971..4033273 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL419_RS20500 (4029567) 4029567..4031717 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NL419_RS20505 (4031767) 4031767..4031970 + 204 WP_000467859.1 YbdD/YjiX family protein -
NL419_RS20510 (4031981) 4031981..4032937 + 957 WP_001297640.1 GTPase -
NL419_RS20515 (4032971) 4032971..4033273 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NL419_RS20520 (4033260) 4033260..4033547 - 288 WP_001360091.1 BrnT family toxin Toxin
NL419_RS20525 (4033746) 4033746..4034443 - 698 Protein_3922 IS1 family transposase -
NL419_RS20530 (4034500) 4034500..4034595 + 96 Protein_3923 dienelactone hydrolase family protein -
NL419_RS20535 (4034741) 4034741..4035973 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
NL419_RS20540 (4036014) 4036014..4037294 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4033746..4033955 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T269446 WP_001360091.1 NZ_CP116923:c4033547-4033260 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT269446 WP_000063156.1 NZ_CP116923:c4033273-4032971 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References