Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2288976..2289502 Replicon chromosome
Accession NZ_CP116923
Organism Escherichia coli strain MLI104K1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NL419_RS11645 Protein ID WP_000323025.1
Coordinates 2289215..2289502 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NL419_RS11640 Protein ID WP_000534858.1
Coordinates 2288976..2289215 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL419_RS11595 (2284211) 2284211..2284632 + 422 Protein_2175 hypothetical protein -
NL419_RS11600 (2285267) 2285267..2285638 + 372 WP_001406656.1 helix-turn-helix domain-containing protein -
NL419_RS11605 (2285676) 2285676..2286338 + 663 Protein_2177 ISNCY family transposase -
NL419_RS11610 (2286459) 2286459..2286608 + 150 WP_011443592.1 protein YdfW -
NL419_RS11615 (2287045) 2287045..2287377 - 333 Protein_2179 FlxA-like family protein -
NL419_RS11620 (2287268) 2287268..2287561 - 294 WP_255055842.1 hypothetical protein -
NL419_RS11625 (2287640) 2287640..2287705 - 66 Protein_2181 hypothetical protein -
NL419_RS11630 (2287764) 2287764..2288732 - 969 WP_012904651.1 IS5 family transposase -
NL419_RS11635 (2288766) 2288766..2288951 - 186 Protein_2183 protein YdfV -
NL419_RS11640 (2288976) 2288976..2289215 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NL419_RS11645 (2289215) 2289215..2289502 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NL419_RS11650 (2289574) 2289574..2289729 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
NL419_RS11655 (2289946) 2289946..2290197 + 252 WP_001463433.1 protein Rem -
NL419_RS11660 (2290264) 2290264..2290542 + 279 WP_001515373.1 hypothetical protein -
NL419_RS11665 (2290544) 2290544..2291593 + 1050 WP_001515372.1 DUF968 domain-containing protein -
NL419_RS11670 (2291611) 2291611..2291922 + 312 WP_072667520.1 antiterminator Q family protein -
NL419_RS11680 (2293405) 2293405..2293929 - 525 WP_000780584.1 lipocalin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2266929..2329351 62422
- inside IScluster/Tn - - 2282938..2294759 11821


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T269439 WP_000323025.1 NZ_CP116923:2289215-2289502 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT269439 WP_000534858.1 NZ_CP116923:2288976-2289215 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References