Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4762738..4763235 Replicon chromosome
Accession NZ_CP116919
Organism Escherichia coli strain MLI102

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL418_RS24125 Protein ID WP_001173343.1
Coordinates 4762738..4762911 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL418_RS24130 Protein ID WP_000398619.1
Coordinates 4762939..4763235 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL418_RS24115 (4759408) 4759408..4760865 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
NL418_RS24120 (4761102) 4761102..4762619 + 1518 WP_001550472.1 lysine--tRNA ligase -
NL418_RS24125 (4762738) 4762738..4762911 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL418_RS24130 (4762939) 4762939..4763235 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL418_RS24135 (4763464) 4763464..4763736 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NL418_RS24140 (4763748) 4763748..4763978 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NL418_RS24145 (4764158) 4764158..4765789 + 1632 WP_001216477.1 sensor histidine kinase -
NL418_RS24150 (4765786) 4765786..4766505 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269427 WP_001173343.1 NZ_CP116919:c4762911-4762738 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269427 WP_000398619.1 NZ_CP116919:c4763235-4762939 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References