Detailed information of TA system TA026942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3134612..3135233
• Replicon: chromosome
• Accession: NC_017530
• Organism: Pseudomonas putida BIRD-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2G8MXB6
• Locus tag: PPUBIRD1_RS26390
• Protein ID: WP_016715573.1
• Coordinates: 3134612..3134794 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PPUBIRD1_RS14085
• Protein ID: WP_014591186.1
• Coordinates: 3134832..3135233 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPUBIRD1_RS27645	3129847..3130008	-	162	WP_085983377.1	hypothetical protein	-
PPUBIRD1_RS14050	3130005..3131684	-	1680	WP_014591182.1	terminase large subunit	-
PPUBIRD1_RS14055	3131688..3132164	-	477	WP_041166996.1	terminase small subunit	-
PPUBIRD1_RS14060	3132321..3132659	-	339	WP_041166997.1	HNH endonuclease	-
PPUBIRD1_RS14065	3132760..3133230	-	471	WP_041166998.1	hypothetical protein	-
PPUBIRD1_RS14070	3133327..3133644	-	318	WP_041166999.1	hypothetical protein	-
PPUBIRD1_RS14075	3133644..3134015	-	372	WP_014591184.1	hypothetical protein	-
PPUBIRD1_RS14080	3134041..3134463	-	423	WP_014591185.1	hypothetical protein	-
PPUBIRD1_RS26390	3134612..3134794	+	183	WP_016715573.1	type II toxin-antitoxin system HicA family toxin	Toxin
PPUBIRD1_RS14085	3134832..3135233	+	402	WP_014591186.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PPUBIRD1_RS14090	3135327..3135887	-	561	WP_041167000.1	hypothetical protein	-
PPUBIRD1_RS14095	3136042..3136431	-	390	WP_041167001.1	antitermination protein Q	-
PPUBIRD1_RS14100	3136443..3136760	-	318	WP_041167002.1	hypothetical protein	-
PPUBIRD1_RS14105	3136757..3137035	-	279	WP_041167003.1	hypothetical protein	-
PPUBIRD1_RS14110	3137032..3138330	-	1299	WP_014591189.1	site-specific integrase	-
PPUBIRD1_RS14115	3138327..3138755	-	429	WP_014591190.1	VRR-NUC domain-containing protein	-
PPUBIRD1_RS14120	3138752..3139048	-	297	WP_014591191.1	DUF1364 domain-containing protein	-
PPUBIRD1_RS14125	3139051..3139860	-	810	WP_014591192.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3097039..3153665	56626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6696.86 Da
• Isoelectric Point: 11.2336

>T26942 WP_016715573.1 NC_017530:3134612-3134794 [Pseudomonas putida BIRD-1]
VQSRQLIKELEAAGWVLKRVTGSHHIFKHPNNPNSIPVPHPKKDLPIGTVKSIKERAGLK

Nucleotide

• Length: 183 bp

>T26942 NC_017530:3134612-3134794 [Pseudomonas putida BIRD-1]
GTGCAAAGCAGGCAGTTGATTAAGGAGCTTGAAGCAGCTGGATGGGTTCTAAAACGTGTCACCGGAAGCCACCACATCTT
CAAACACCCCAACAACCCAAACTCAATACCAGTGCCACACCCCAAAAAGGATCTACCGATCGGCACGGTAAAGAGCATCA
AGGAAAGAGCCGGGTTGAAATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14497.42 Da
• Isoelectric Point: 4.2687

>AT26942 WP_014591186.1 NC_017530:3134832-3135233 [Pseudomonas putida BIRD-1]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDTFEETYASAVEVAHIMLEEIAGSGQAIPMPTSAAAHRSNPDFAEMGWGM
LEIDITPYLGKTEKVNVTLPGFVIQQIDRYVRDHNVKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT26942 NC_017530:3134832-3135233 [Pseudomonas putida BIRD-1]
ATGCAATACCCAATCTGCATCGAGTGGGGCGACGAAAACACCGCCATCGGCATTCAGATCCCCGACATTCCTGGCGCTGT
GACAGCTGGCGACACCTTCGAGGAGACCTATGCCTCAGCTGTCGAGGTCGCCCACATCATGCTGGAAGAGATCGCTGGCA
GTGGCCAGGCTATTCCCATGCCGACCAGCGCTGCAGCACACCGGAGCAATCCCGACTTCGCCGAAATGGGATGGGGCATG
CTGGAAATCGACATCACGCCGTACCTGGGCAAGACCGAGAAGGTCAATGTCACCCTGCCGGGATTCGTTATCCAGCAGAT
TGACCGATACGTACGCGATCACAACGTGAAGAGCCGGTCTTCTTTCCTAGCCGATGCAGCCATGGAAAAGCTGGGCCGCT
AG

Structures

Toxin

• AlphaFold DB: A0A2G8MXB6