Detailed information of TA system TA026933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3132942..3133546
• Replicon: chromosome
• Accession: NC_017524
• Organism: Mycobacterium tuberculosis CTRI-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: MTCTRI2_RS14920
• Protein ID: WP_003414492.1
• Coordinates: 3132942..3133334 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: MTCTRI2_RS14925
• Protein ID: WP_003414495.1
• Coordinates: 3133331..3133546 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTCTRI2_RS14890	3128092..3128880	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
MTCTRI2_RS14895	3129214..3129759	-	546	WP_003913758.1	DUF1802 family protein	-
MTCTRI2_RS14900	3130031..3130915	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MTCTRI2_RS14905	3130918..3131805	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
MTCTRI2_RS14910	3132110..3132655	-	546	WP_003899500.1	DUF1802 family protein	-
MTCTRI2_RS14915	3132652..3132921	-	270	WP_003414489.1	DUF2277 family protein	-
MTCTRI2_RS14920	3132942..3133334	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
MTCTRI2_RS14925	3133331..3133546	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MTCTRI2_RS14930	3133593..3134342	+	750	WP_003414497.1	enoyl-CoA hydratase	-
MTCTRI2_RS14935	3134421..3135503	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
MTCTRI2_RS14940	3135496..3136806	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
MTCTRI2_RS14945	3136809..3137636	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
MTCTRI2_RS14950	3137633..3138544	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T26933 WP_003414492.1 NC_017524:c3133334-3132942 [Mycobacterium tuberculosis CTRI-2]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T26933 NC_017524:c3133334-3132942 [Mycobacterium tuberculosis CTRI-2]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT26933 WP_003414495.1 NC_017524:c3133546-3133331 [Mycobacterium tuberculosis CTRI-2]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT26933 NC_017524:c3133546-3133331 [Mycobacterium tuberculosis CTRI-2]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8