Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3103868..3104438 Replicon chromosome
Accession NC_017524
Organism Mycobacterium tuberculosis CTRI-2

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag MTCTRI2_RS14750 Protein ID WP_003414166.1
Coordinates 3103868..3104224 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag MTCTRI2_RS14755 Protein ID WP_003901465.1
Coordinates 3104208..3104438 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTCTRI2_RS14730 3099320..3101008 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
MTCTRI2_RS14735 3101012..3101338 - 327 WP_003414157.1 hypothetical protein -
MTCTRI2_RS14740 3101511..3102098 + 588 WP_003914429.1 DUF3558 family protein -
MTCTRI2_RS14745 3102117..3103766 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
MTCTRI2_RS14750 3103868..3104224 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
MTCTRI2_RS14755 3104208..3104438 - 231 WP_003901465.1 antitoxin MazE Antitoxin
MTCTRI2_RS14760 3104481..3105524 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
MTCTRI2_RS22195 3105715..3105990 + 276 WP_003911993.1 DUF1778 domain-containing protein -
MTCTRI2_RS22540 3106166..3106420 - 255 WP_003917684.1 hypothetical protein -
MTCTRI2_RS14775 3106568..3106972 + 405 WP_009938577.1 hypothetical protein -
MTCTRI2_RS14780 3106969..3107160 + 192 WP_003414184.1 hypothetical protein -
MTCTRI2_RS14785 3107224..3108513 + 1290 Protein_2897 transposase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3093903..3118719 24816


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T26931 WP_003414166.1 NC_017524:c3104224-3103868 [Mycobacterium tuberculosis CTRI-2]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T26931 NC_017524:c3104224-3103868 [Mycobacterium tuberculosis CTRI-2]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT26931 WP_003901465.1 NC_017524:c3104438-3104208 [Mycobacterium tuberculosis CTRI-2]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT26931 NC_017524:c3104438-3104208 [Mycobacterium tuberculosis CTRI-2]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References