Detailed information of TA system TA026930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3064284..3064971
• Replicon: chromosome
• Accession: NC_017524
• Organism: Mycobacterium tuberculosis CTRI-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: MTCTRI2_RS14540
• Protein ID: WP_003414064.1
• Coordinates: 3064576..3064971 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: MTCTRI2_RS14535
• Protein ID: WP_003414061.1
• Coordinates: 3064284..3064550 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTCTRI2_RS14510	3059923..3060825	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MTCTRI2_RS14515	3060894..3061646	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
MTCTRI2_RS14520	3061890..3062165	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
MTCTRI2_RS14525	3062162..3063784	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
MTCTRI2_RS14530	3063871..3064287	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
MTCTRI2_RS14535	3064284..3064550	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MTCTRI2_RS14540	3064576..3064971	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
MTCTRI2_RS14545	3064968..3065237	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
MTCTRI2_RS14550	3065247..3066341	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
MTCTRI2_RS14555	3066338..3066757	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
MTCTRI2_RS14560	3066831..3067310	-	480	WP_003414073.1	dihydrofolate reductase	-
MTCTRI2_RS14565	3067381..3068181	-	801	WP_009939104.1	thymidylate synthase	-
MTCTRI2_RS14570	3068337..3069074	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T26930 WP_003414064.1 NC_017524:c3064971-3064576 [Mycobacterium tuberculosis CTRI-2]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T26930 NC_017524:c3064971-3064576 [Mycobacterium tuberculosis CTRI-2]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT26930 WP_003414061.1 NC_017524:c3064550-3064284 [Mycobacterium tuberculosis CTRI-2]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT26930 NC_017524:c3064550-3064284 [Mycobacterium tuberculosis CTRI-2]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64