Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518664..519300 Replicon chromosome
Accession NZ_CP116870
Organism Bacillus subtilis subsp. subtilis strain Master_strain

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PPM45_RS02645 Protein ID WP_003156187.1
Coordinates 518950..519300 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag PPM45_RS02640 Protein ID WP_003225183.1
Coordinates 518664..518945 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPM45_RS02620 515023..515622 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
PPM45_RS02625 515717..516082 + 366 WP_003234281.1 holo-ACP synthase -
PPM45_RS02630 516248..517264 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
PPM45_RS02635 517379..518548 + 1170 WP_003234284.1 alanine racemase -
PPM45_RS02640 518664..518945 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PPM45_RS02645 518950..519300 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PPM45_RS02650 519415..520239 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
PPM45_RS02655 520244..520609 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
PPM45_RS02660 520613..521014 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
PPM45_RS02665 521026..522033 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
PPM45_RS02670 522095..522424 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
PPM45_RS02675 522421..522903 + 483 WP_003234299.1 anti-sigma B factor RsbW -
PPM45_RS02680 522869..523657 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
PPM45_RS02685 523657..524256 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T269296 WP_003156187.1 NZ_CP116870:518950-519300 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT269296 WP_003225183.1 NZ_CP116870:518664-518945 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References