Detailed information of TA system TA269280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 387982..388618
• Replicon: chromosome
• Accession: NZ_CP116866
• Organism: Bacillus subtilis subsp. subtilis strain MGP013

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: PPM57_RS01995
• Protein ID: WP_003156187.1
• Coordinates: 388268..388618 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: PPM57_RS01990
• Protein ID: WP_003225183.1
• Coordinates: 387982..388263 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPM57_RS01970	384341..384940	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
PPM57_RS01975	385035..385400	+	366	WP_003234281.1	holo-ACP synthase	-
PPM57_RS01980	385566..386582	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
PPM57_RS01985	386697..387866	+	1170	WP_003234284.1	alanine racemase	-
PPM57_RS01990	387982..388263	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
PPM57_RS01995	388268..388618	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
PPM57_RS02000	388733..389557	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
PPM57_RS02005	389562..389927	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
PPM57_RS02010	389931..390332	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
PPM57_RS02015	390344..391351	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
PPM57_RS02020	391413..391742	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
PPM57_RS02025	391739..392221	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
PPM57_RS02030	392187..392975	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
PPM57_RS02035	392975..393574	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T269280 WP_003156187.1 NZ_CP116866:388268-388618 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T269280 NZ_CP116866:388268-388618 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT269280 WP_003225183.1 NZ_CP116866:387982-388263 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT269280 NZ_CP116866:387982-388263 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32