Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 310696..311332 Replicon chromosome
Accession NZ_CP116864
Organism Bacillus subtilis subsp. subtilis strain MGP022

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PPM28_RS01585 Protein ID WP_003156187.1
Coordinates 310982..311332 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag PPM28_RS01580 Protein ID WP_003225183.1
Coordinates 310696..310977 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPM28_RS01560 307055..307654 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
PPM28_RS01565 307749..308114 + 366 WP_003234281.1 holo-ACP synthase -
PPM28_RS01570 308280..309296 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
PPM28_RS01575 309411..310580 + 1170 WP_003234284.1 alanine racemase -
PPM28_RS01580 310696..310977 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PPM28_RS01585 310982..311332 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PPM28_RS01590 311447..312271 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
PPM28_RS01595 312276..312641 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
PPM28_RS01600 312645..313046 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
PPM28_RS01605 313058..314065 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
PPM28_RS01610 314127..314456 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
PPM28_RS01615 314453..314935 + 483 WP_003234299.1 anti-sigma B factor RsbW -
PPM28_RS01620 314901..315689 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
PPM28_RS01625 315689..316288 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T269272 WP_003156187.1 NZ_CP116864:310982-311332 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT269272 WP_003225183.1 NZ_CP116864:310696-310977 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References