Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2864630..2865267 Replicon chromosome
Accession NC_017524
Organism Mycobacterium tuberculosis CTRI-2

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag MTCTRI2_RS13465 Protein ID WP_003413180.1
Coordinates 2864630..2865025 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag MTCTRI2_RS13470 Protein ID WP_003413183.1
Coordinates 2865022..2865267 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTCTRI2_RS13425 2860033..2861244 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
MTCTRI2_RS13430 2861371..2862030 + 660 WP_014585545.1 LppA family lipoprotein -
MTCTRI2_RS13435 2862027..2862689 + 663 WP_003900848.1 hypothetical protein -
MTCTRI2_RS13440 2862686..2862964 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
MTCTRI2_RS13445 2863057..2863470 + 414 WP_003413164.1 PIN domain nuclease -
MTCTRI2_RS13450 2863509..2863766 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
MTCTRI2_RS13455 2863763..2864140 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
MTCTRI2_RS13460 2864156..2864530 - 375 WP_003413177.1 hypothetical protein -
MTCTRI2_RS13465 2864630..2865025 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
MTCTRI2_RS13470 2865022..2865267 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
MTCTRI2_RS13475 2865678..2866097 - 420 WP_003413190.1 A24 family peptidase -
MTCTRI2_RS13480 2866109..2866918 - 810 WP_003413193.1 shikimate dehydrogenase -
MTCTRI2_RS13485 2866915..2868168 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MTCTRI2_RS13490 2868161..2868673 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T26926 WP_003413180.1 NC_017524:c2865025-2864630 [Mycobacterium tuberculosis CTRI-2]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T26926 NC_017524:c2865025-2864630 [Mycobacterium tuberculosis CTRI-2]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT26926 WP_003413183.1 NC_017524:c2865267-2865022 [Mycobacterium tuberculosis CTRI-2]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT26926 NC_017524:c2865267-2865022 [Mycobacterium tuberculosis CTRI-2]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References