Detailed information of TA system TA269245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 5960662..5962340
• Replicon: chromosome
• Accession: NZ_CP116775
• Organism: Pseudomonas sp. 273

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: PKO50_RS27235
• Protein ID: WP_275627786.1
• Coordinates: 5960662..5961522 (+)
• Length: 287 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: PKO50_RS27240
• Protein ID: WP_089393418.1
• Coordinates: 5961519..5962340 (+)
• Length: 274 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PKO50_RS27210	5956664..5957524	+	861	WP_275627784.1	ABC transporter ATP-binding protein	-
PKO50_RS27215	5957546..5958544	+	999	WP_109936708.1	ABC transporter substrate-binding protein	-
PKO50_RS27220	5958561..5959333	+	773	Protein_5381	ABC transporter permease	-
PKO50_RS27225	5959344..5960105	+	762	WP_275627785.1	SDR family oxidoreductase	-
PKO50_RS27230	5960118..5960651	+	534	WP_109936711.1	cupin domain-containing protein	-
PKO50_RS27235	5960662..5961522	+	861	WP_275627786.1	alpha/beta fold hydrolase	Toxin
PKO50_RS27240	5961519..5962340	+	822	WP_089393418.1	IclR family transcriptional regulator	Antitoxin
PKO50_RS27245	5962337..5963134	+	798	WP_275627787.1	SDR family oxidoreductase	-
PKO50_RS27250	5963127..5964808	+	1682	Protein_5387	thiamine pyrophosphate-binding protein	-
PKO50_RS27255	5964819..5965622	+	804	WP_109936715.1	aspartate dehydrogenase	-
PKO50_RS27260	5965633..5967120	+	1488	WP_275627788.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (43-271)

Antitoxin

• Transcription regulator IclR, N-terminal (18-67)

Sequences

Toxin

Protein

• Length: 287 a.a.
• Molecular weight: 30094.73 Da
• Isoelectric Point: 7.3293

>T269245 WP_275627786.1 NZ_CP116775:5960662-5961522 [Pseudomonas sp. 273]
MTAMTAAAAWQADLERDFPQRLVRHGETVAAVREAGATDAPALVLLHGIGSGAASWLPLAQRLKDRARIIAWDAPGYGES
TPLAEAAPRAEAYMGRLRELLDALGVRRCLLLGHSLGAMAALALANAEPQRVARLVLLSPARGYGARPDEGEAVRSRRLA
GLASEGIAGLAERRAPAMLGANPDAAALAWVRWNMARLRPHGYRQAVELLCGDDLLRLGRPPVPSEVLCGSEDGITPPAA
CREVAEALAAPFQLVPGAGHASPIECPEALAGLLARLIEHCQGLKP

Nucleotide

• Length: 861 bp

Antitoxin

Protein

• Length: 274 a.a.
• Molecular weight: 30318.93 Da
• Isoelectric Point: 6.7546

>AT269245 WP_089393418.1 NZ_CP116775:5961519-5962340 [Pseudomonas sp. 273]
MSTDTLNEPQERYMVPGLERGLTLLGEFTRHDRTLTAPELARRLQLPRTTIFRLLATLEALGFVTRSGNEYRLGMAVLRL
GFEYLASLELTELGQPILRRLCDAIGYSCNLVVRDGRNIVYVAKISPPSILTSAVNVGTRLPAHATVLGRILLEDLDLAA
LRELYPEKQLERFSEGTPRNAEELFDLVQNDRLRGYALSEGFFEASISTVAAPVRDHTGRVCAAMGVTIPATRIAEHLLE
PLVRQVRNSAEELSGLLGHMPNPNVLSLQRVHP

Nucleotide

• Length: 822 bp