Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3239261..3239897 Replicon chromosome
Accession NZ_CP116774
Organism Bacillus safensis strain SRCM125915

Toxin (Protein)


Gene name mazF Uniprot ID A0A0P7G713
Locus tag PNF30_RS16715 Protein ID WP_024425388.1
Coordinates 3239261..3239611 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag PNF30_RS16720 Protein ID WP_003214273.1
Coordinates 3239616..3239897 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PNF30_RS16675 (PNF30_16675) 3234291..3234890 - 600 WP_205183263.1 PP2C family serine/threonine-protein phosphatase -
PNF30_RS16680 (PNF30_16680) 3234890..3235678 - 789 WP_024427378.1 RNA polymerase sigma factor SigB -
PNF30_RS16685 (PNF30_16685) 3235644..3236132 - 489 WP_048240335.1 anti-sigma B factor RsbW -
PNF30_RS16690 (PNF30_16690) 3236129..3236458 - 330 WP_017358393.1 anti-sigma factor antagonist -
PNF30_RS16695 (PNF30_16695) 3236518..3237525 - 1008 WP_095407804.1 PP2C family protein-serine/threonine phosphatase -
PNF30_RS16700 (PNF30_16700) 3237536..3237937 - 402 WP_024427376.1 anti-sigma regulatory factor -
PNF30_RS16705 (PNF30_16705) 3237940..3238308 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
PNF30_RS16710 (PNF30_16710) 3238313..3239143 - 831 WP_272510456.1 RsbT co-antagonist protein RsbRA -
PNF30_RS16715 (PNF30_16715) 3239261..3239611 - 351 WP_024425388.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PNF30_RS16720 (PNF30_16720) 3239616..3239897 - 282 WP_003214273.1 hypothetical protein Antitoxin
PNF30_RS16725 (PNF30_16725) 3240191..3241357 - 1167 WP_272510457.1 alanine racemase -
PNF30_RS16730 (PNF30_16730) 3241497..3242513 - 1017 WP_095407802.1 outer membrane lipoprotein carrier protein LolA -
PNF30_RS16735 (PNF30_16735) 3242674..3243039 - 366 WP_272510458.1 holo-ACP synthase -
PNF30_RS16740 (PNF30_16740) 3243134..3243739 + 606 WP_144472946.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8891

>T269232 WP_024425388.1 NZ_CP116774:c3239611-3239261 [Bacillus safensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT269232 WP_003214273.1 NZ_CP116774:c3239897-3239616 [Bacillus safensis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P7G713

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References