Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3542030..3542666 Replicon chromosome
Accession NZ_CP116773
Organism Bacillus subtilis strain SRCM125727

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PNF29_RS18800 Protein ID WP_003156187.1
Coordinates 3542030..3542380 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag PNF29_RS18805 Protein ID WP_003225183.1
Coordinates 3542385..3542666 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PNF29_RS18760 (PNF29_18760) 3537074..3537673 - 600 WP_017696999.1 phosphoserine phosphatase RsbX -
PNF29_RS18765 (PNF29_18765) 3537673..3538461 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
PNF29_RS18770 (PNF29_18770) 3538427..3538909 - 483 WP_003234299.1 anti-sigma B factor RsbW -
PNF29_RS18775 (PNF29_18775) 3538906..3539235 - 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
PNF29_RS18780 (PNF29_18780) 3539297..3540304 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
PNF29_RS18785 (PNF29_18785) 3540316..3540717 - 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
PNF29_RS18790 (PNF29_18790) 3540721..3541086 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
PNF29_RS18795 (PNF29_18795) 3541091..3541915 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
PNF29_RS18800 (PNF29_18800) 3542030..3542380 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PNF29_RS18805 (PNF29_18805) 3542385..3542666 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PNF29_RS18810 (PNF29_18810) 3542782..3543951 - 1170 WP_017697002.1 alanine racemase -
PNF29_RS18815 (PNF29_18815) 3544065..3545081 - 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
PNF29_RS18820 (PNF29_18820) 3545247..3545612 - 366 WP_014475874.1 holo-ACP synthase -
PNF29_RS18825 (PNF29_18825) 3545707..3546306 + 600 WP_041351790.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T269231 WP_003156187.1 NZ_CP116773:c3542380-3542030 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT269231 WP_003225183.1 NZ_CP116773:c3542666-3542385 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References