269230

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 2691337..2692253 Replicon chromosome
Accession NZ_CP116773
Organism Bacillus subtilis strain SRCM125727

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag PNF29_RS14260 Protein ID WP_003244695.1
Coordinates 2691337..2692083 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag PNF29_RS14265 Protein ID WP_003232646.1
Coordinates 2692083..2692253 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PNF29_RS14235 (PNF29_14235) 2686455..2686556 - 102 Protein_2809 hypothetical protein -
PNF29_RS14240 (PNF29_14240) 2686665..2687615 - 951 WP_014476561.1 ring-cleaving dioxygenase -
PNF29_RS14245 (PNF29_14245) 2688004..2689320 + 1317 WP_272515589.1 serine/threonine exchanger -
PNF29_RS14250 (PNF29_14250) 2689596..2690213 + 618 WP_003218470.1 DUF47 domain-containing protein -
PNF29_RS14255 (PNF29_14255) 2690226..2691227 + 1002 WP_014479569.1 inorganic phosphate transporter -
PNF29_RS14260 (PNF29_14260) 2691337..2692083 + 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
PNF29_RS14265 (PNF29_14265) 2692083..2692253 + 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
PNF29_RS14270 (PNF29_14270) 2692339..2692476 + 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
PNF29_RS14275 (PNF29_14275) 2692514..2693407 - 894 WP_041333865.1 N-acetylmuramoyl-L-alanine amidase -
PNF29_RS14280 (PNF29_14280) 2693420..2693683 - 264 WP_003232653.1 phage holin -
PNF29_RS14285 (PNF29_14285) 2693696..2693965 - 270 WP_003232655.1 hemolysin XhlA family protein -
PNF29_RS14290 (PNF29_14290) 2694018..2694857 - 840 WP_128473844.1 phage-like element PBSX protein XepA -
PNF29_RS14295 (PNF29_14295) 2694901..2695065 - 165 WP_087960875.1 XkdX family protein -
PNF29_RS14300 (PNF29_14300) 2695062..2695391 - 330 WP_003232660.1 XkdW family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T269230 WP_003244695.1 NZ_CP116773:2691337-2692083 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT269230 WP_003232646.1 NZ_CP116773:2692083-2692253 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References