Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 400958..401748 Replicon plasmid pAtFPH-AT4a
Accession NZ_CP116732
Organism Agrobacterium fabacearum strain FPH-AT4

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1S7NFF1
Locus tag G6L23_RS24850 Protein ID WP_019565755.1
Coordinates 401251..401748 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A1S7NER6
Locus tag G6L23_RS24845 Protein ID WP_019565754.1
Coordinates 400958..401254 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6L23_RS24825 (G6L23_024825) 396326..398050 + 1725 WP_013637436.1 ABC transporter ATP-binding protein -
G6L23_RS24830 (G6L23_024830) 398095..399282 + 1188 WP_145166916.1 galactarate dehydratase -
G6L23_RS24835 (G6L23_024835) 399269..400135 + 867 WP_174048019.1 aldose 1-epimerase -
G6L23_RS24840 (G6L23_024840) 400345..400775 + 431 Protein_410 DDE-type integrase/transposase/recombinase -
G6L23_RS24845 (G6L23_024845) 400958..401254 + 297 WP_019565754.1 DUF1778 domain-containing protein Antitoxin
G6L23_RS24850 (G6L23_024850) 401251..401748 + 498 WP_019565755.1 GNAT family N-acetyltransferase Toxin
G6L23_RS24855 (G6L23_024855) 402367..402915 + 549 WP_019565757.1 TRAP transporter small permease subunit -
G6L23_RS24860 (G6L23_024860) 402933..404438 + 1506 WP_174047949.1 TRAP transporter large permease subunit -
G6L23_RS24865 (G6L23_024865) 404502..405614 + 1113 WP_065703185.1 TRAP transporter substrate-binding protein -
G6L23_RS24870 (G6L23_024870) 405678..406310 - 633 WP_019565760.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..735041 735041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-142)

Antitoxin

Vibrio phage ICP1, Orf50

(16-90)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 17725.27 Da        Isoelectric Point: 6.8629

>T269219 WP_019565755.1 NZ_CP116732:401251-401748 [Agrobacterium fabacearum]
VTLSAPVPLADHHELAEFNSGVPELDDWLRRRARANQAGGASRTFVVCDESRVIAYYALASGSVKPPEVPGRFRRNMPDP
IPVAVLGRLAIDQSCQGRGIGRALVRDAGLRLLNAAEVLGIRGVLVHAISDDARAFYEAVGFLSSPSDPMMLMVGLHDLN
NALNT

Download         Length: 498 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11022.59 Da        Isoelectric Point: 7.0159

>AT269219 WP_019565754.1 NZ_CP116732:400958-401254 [Agrobacterium fabacearum]
MPTSHTGKSPDASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIVTVSPEIYAEYLARLDAPAQ
PNERLKRTMSTKAPWDEA

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S7NFF1

Antitoxin

Source ID Structure
AlphaFold DB A0A1S7NER6

References