Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2227869..2228455 Replicon chromosome
Accession NC_017524
Organism Mycobacterium tuberculosis CTRI-2

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag MTCTRI2_RS10435 Protein ID WP_003410010.1
Coordinates 2227869..2228213 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag MTCTRI2_RS10440 Protein ID WP_003410014.1
Coordinates 2228207..2228455 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTCTRI2_RS10400 2223575..2224174 + 600 WP_003409989.1 L-lysine exporter -
MTCTRI2_RS10405 2224590..2225018 + 429 WP_003906713.1 cellulose-binding protein -
MTCTRI2_RS10410 2225244..2225783 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
MTCTRI2_RS10415 2226303..2226863 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
MTCTRI2_RS10420 2226860..2227201 - 342 WP_003410003.1 DUF2384 domain-containing protein -
MTCTRI2_RS10425 2227287..2227544 + 258 WP_003410006.1 hypothetical protein -
MTCTRI2_RS10430 2227445..2227780 - 336 WP_003410009.1 dehydrogenase -
MTCTRI2_RS10435 2227869..2228213 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
MTCTRI2_RS10440 2228207..2228455 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
MTCTRI2_RS10445 2228555..2230870 - 2316 WP_003899120.1 cation transporter ATPase CptG -
MTCTRI2_RS10450 2230867..2231139 - 273 WP_003410017.1 DUF1490 family protein -
MTCTRI2_RS10455 2231192..2231548 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
MTCTRI2_RS10460 2231705..2232472 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T26916 WP_003410010.1 NC_017524:c2228213-2227869 [Mycobacterium tuberculosis CTRI-2]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T26916 NC_017524:c2228213-2227869 [Mycobacterium tuberculosis CTRI-2]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT26916 WP_003410014.1 NC_017524:c2228455-2228207 [Mycobacterium tuberculosis CTRI-2]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT26916 NC_017524:c2228455-2228207 [Mycobacterium tuberculosis CTRI-2]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References