Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 300463..301253 Replicon plasmid pAtA47a_a
Accession NZ_CP116702
Organism Agrobacterium fabacearum strain A47a

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1S7NFF1
Locus tag G6M09_RS24170 Protein ID WP_019565755.1
Coordinates 300756..301253 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A1S7NER6
Locus tag G6M09_RS24165 Protein ID WP_019565754.1
Coordinates 300463..300759 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M09_RS24145 (G6M09_024145) 295831..297555 + 1725 WP_019565751.1 ABC transporter ATP-binding protein -
G6M09_RS24150 (G6M09_024150) 297600..298787 + 1188 WP_145166916.1 galactarate dehydratase -
G6M09_RS24155 (G6M09_024155) 298774..299640 + 867 WP_145166983.1 aldose 1-epimerase -
G6M09_RS24160 (G6M09_024160) 299850..300280 + 431 Protein_297 DDE-type integrase/transposase/recombinase -
G6M09_RS24165 (G6M09_024165) 300463..300759 + 297 WP_019565754.1 DUF1778 domain-containing protein Antitoxin
G6M09_RS24170 (G6M09_024170) 300756..301253 + 498 WP_019565755.1 GNAT family N-acetyltransferase Toxin
G6M09_RS24175 (G6M09_024175) 301872..302420 + 549 WP_019565757.1 TRAP transporter small permease subunit -
G6M09_RS24180 (G6M09_024180) 302438..303943 + 1506 WP_173995354.1 TRAP transporter large permease subunit -
G6M09_RS24185 (G6M09_024185) 304007..305119 + 1113 WP_065703185.1 TRAP transporter substrate-binding protein -
G6M09_RS24190 (G6M09_024190) 305183..305815 - 633 WP_173995355.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..565096 565096


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-142)

Antitoxin

Vibrio phage ICP1, Orf50

(16-90)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 17725.27 Da        Isoelectric Point: 6.8629

>T269142 WP_019565755.1 NZ_CP116702:300756-301253 [Agrobacterium fabacearum]
VTLSAPVPLADHHELAEFNSGVPELDDWLRRRARANQAGGASRTFVVCDESRVIAYYALASGSVKPPEVPGRFRRNMPDP
IPVAVLGRLAIDQSCQGRGIGRALVRDAGLRLLNAAEVLGIRGVLVHAISDDARAFYEAVGFLSSPSDPMMLMVGLHDLN
NALNT

Download         Length: 498 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11022.59 Da        Isoelectric Point: 7.0159

>AT269142 WP_019565754.1 NZ_CP116702:300463-300759 [Agrobacterium fabacearum]
MPTSHTGKSPDASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIVTVSPEIYAEYLARLDAPAQ
PNERLKRTMSTKAPWDEA

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S7NFF1

Antitoxin

Source ID Structure
AlphaFold DB A0A1S7NER6

References