Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 16243..16939 Replicon plasmid pK205-3-A
Accession NZ_CP116576
Organism Enterococcus faecalis strain K205-3

Toxin (Protein)


Gene name zeta Uniprot ID A0A828ZQ58
Locus tag PML83_RS14300 Protein ID WP_002334977.1
Coordinates 16517..16939 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag PML83_RS14295 Protein ID WP_002331065.1
Coordinates 16243..16515 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML83_RS14265 (PML83_14265) 11542..12450 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
PML83_RS14270 (PML83_14270) 12447..12989 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
PML83_RS14275 (PML83_14275) 13082..13876 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
PML83_RS14280 (PML83_14280) 14152..14724 + 573 WP_001079938.1 HTH domain-containing protein -
PML83_RS14285 (PML83_14285) 15022..15918 + 897 WP_002334978.1 AAA family ATPase -
PML83_RS14290 (PML83_14290) 16017..16226 + 210 WP_000527318.1 peptide-binding protein -
PML83_RS14295 (PML83_14295) 16243..16515 + 273 WP_002331065.1 antitoxin Antitoxin
PML83_RS14300 (PML83_14300) 16517..16939 + 423 WP_002334977.1 zeta toxin family protein Toxin
PML83_RS14305 (PML83_14305) 16993..17673 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
PML83_RS14310 (PML83_14310) 17742..17936 - 195 WP_002368123.1 hypothetical protein -
PML83_RS14315 (PML83_14315) 18172..18495 + 324 WP_002360731.1 hypothetical protein -
PML83_RS14320 (PML83_14320) 18756..19415 - 660 WP_002368120.1 hypothetical protein -
PML83_RS14325 (PML83_14325) 19914..20288 - 375 WP_010816301.1 DUF805 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / aph(3')-III - 1..40660 40660
- inside IScluster/Tn ant(6)-Ia / aph(3')-III - 470..17673 17203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-134)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15667.64 Da        Isoelectric Point: 5.2420

>T269050 WP_002334977.1 NZ_CP116576:16517-16939 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSGSVAKF

Download         Length: 423 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT269050 WP_002331065.1 NZ_CP116576:16243-16515 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZQ58

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References