Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 5450..6587 Replicon plasmid pK190-1-B
Accession NZ_CP116573
Organism Enterococcus faecalis strain K190-1

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag PML73_RS14795 Protein ID WP_002332783.1
Coordinates 5724..6587 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag PML73_RS14790 Protein ID WP_002326825.1
Coordinates 5450..5722 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML73_RS14750 (PML73_14750) 657..1487 + 831 WP_000242080.1 undecaprenyl-diphosphate phosphatase -
PML73_RS14755 (PML73_14755) 1684..2364 - 681 WP_000191454.1 IS6-like element ISEnfa1 family transposase -
PML73_RS14760 (PML73_14760) 2463..2702 + 240 WP_000635249.1 peptide-binding protein -
PML73_RS14765 (PML73_14765) 2751..2834 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
PML73_RS14770 (PML73_14770) 2959..3696 + 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PML73_RS14775 (PML73_14775) 3866..4126 + 261 Protein_6 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
PML73_RS14780 (PML73_14780) 4229..5125 + 897 WP_002326827.1 ParA family protein -
PML73_RS14785 (PML73_14785) 5217..5432 + 216 WP_001835296.1 peptide-binding protein -
PML73_RS14790 (PML73_14790) 5450..5722 + 273 WP_002326825.1 antitoxin Antitoxin
PML73_RS14795 (PML73_14795) 5724..6587 + 864 WP_002332783.1 zeta toxin family protein Toxin
PML73_RS14800 (PML73_14800) 7027..7344 + 318 WP_002326830.1 hypothetical protein -
PML73_RS14805 (PML73_14805) 7878..8375 + 498 WP_002338758.1 hypothetical protein -
PML73_RS14810 (PML73_14810) 8409..8642 + 234 WP_002332654.1 hypothetical protein -
PML73_RS14815 (PML73_14815) 8736..8993 - 258 WP_000002668.1 hypothetical protein -
PML73_RS14820 (PML73_14820) 8996..9295 - 300 WP_002332653.1 hypothetical protein -
PML73_RS14825 (PML73_14825) 9578..11572 + 1995 WP_229205849.1 MobA/MobL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / aac(6')-aph(2'') - 1..38206 38206
- flank IS/Tn erm(B) - 1684..3696 2012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T269027 WP_002332783.1 NZ_CP116573:5724-6587 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT269027 WP_002326825.1 NZ_CP116573:5450-5722 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References