Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1231022..1231653 Replicon chromosome
Accession NC_017524
Organism Mycobacterium tuberculosis CTRI-2

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag MTCTRI2_RS05865 Protein ID WP_003898728.1
Coordinates 1231022..1231333 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag MTCTRI2_RS05870 Protein ID WP_003405836.1
Coordinates 1231333..1231653 (-) Length 107 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTCTRI2_RS05845 1226503..1227927 - 1425 WP_003405805.1 class II fumarate hydratase -
MTCTRI2_RS05850 1227958..1229046 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
MTCTRI2_RS05855 1229102..1229746 + 645 WP_003915494.1 DUF4245 domain-containing protein -
MTCTRI2_RS05860 1229753..1230910 - 1158 WP_003898727.1 AI-2E family transporter -
MTCTRI2_RS05865 1231022..1231333 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
MTCTRI2_RS05870 1231333..1231653 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
MTCTRI2_RS21005 1231663..1233188 + 1526 Protein_1147 carboxylesterase/lipase family protein -
MTCTRI2_RS05885 1233206..1234318 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
MTCTRI2_RS05890 1234328..1234585 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
MTCTRI2_RS05895 1234575..1235822 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
MTCTRI2_RS05900 1235819..1236457 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-85)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T26901 WP_003898728.1 NC_017524:c1231333-1231022 [Mycobacterium tuberculosis CTRI-2]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T26901 NC_017524:c1231333-1231022 [Mycobacterium tuberculosis CTRI-2]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT26901 WP_003405836.1 NC_017524:c1231653-1231333 [Mycobacterium tuberculosis CTRI-2]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT26901 NC_017524:c1231653-1231333 [Mycobacterium tuberculosis CTRI-2]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT


Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References