268955

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 21520..22657 Replicon plasmid pK188-1-A
Accession NZ_CP116565
Organism Enterococcus faecalis strain K188-1

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag PML72_RS13905 Protein ID WP_002332783.1
Coordinates 21794..22657 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag PML72_RS13900 Protein ID WP_002326825.1
Coordinates 21520..21792 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML72_RS13860 (PML72_13860) 16536..16619 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
PML72_RS13865 (PML72_13865) 16744..17481 + 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PML72_RS13870 (PML72_13870) 17485..18279 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
PML72_RS13875 (PML72_13875) 18821..18904 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
PML72_RS13880 (PML72_13880) 19029..19766 + 738 WP_001038789.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PML72_RS13885 (PML72_13885) 19936..20196 + 261 Protein_21 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
PML72_RS13890 (PML72_13890) 20299..21195 + 897 WP_002326827.1 ParA family protein -
PML72_RS13895 (PML72_13895) 21287..21502 + 216 WP_001835296.1 peptide-binding protein -
PML72_RS13900 (PML72_13900) 21520..21792 + 273 WP_002326825.1 antitoxin Antitoxin
PML72_RS13905 (PML72_13905) 21794..22657 + 864 WP_002332783.1 zeta toxin family protein Toxin
PML72_RS13910 (PML72_13910) 23097..23414 + 318 WP_002326830.1 hypothetical protein -
PML72_RS13915 (PML72_13915) 23614..23910 + 297 WP_002311824.1 hypothetical protein -
PML72_RS13920 (PML72_13920) 23917..25869 + 1953 WP_000163792.1 hypothetical protein -
PML72_RS13925 (PML72_13925) 25941..26438 - 498 WP_000868795.1 trimethoprim-resistant dihydrofolate reductase DfrG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(L) / tet(M) / erm(B) / aph(3')-III / dfrG / fexA / optrA / erm(A) bsh 1..74469 74469
- flank IS/Tn erm(B) / aph(3')-III - 15275..19766 4491


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T268955 WP_002332783.1 NZ_CP116565:21794-22657 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT268955 WP_002326825.1 NZ_CP116565:21520-21792 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References