Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 31313..32450 Replicon plasmid pK80-15b-B
Accession NZ_CP116551
Organism Enterococcus faecium strain K80-15b

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag PML85_RS13925 Protein ID WP_010717401.1
Coordinates 31587..32450 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag PML85_RS13920 Protein ID WP_000301765.1
Coordinates 31313..31585 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML85_RS13890 (PML85_13890) 28297..28536 + 240 WP_002425012.1 peptide-binding protein -
PML85_RS13895 (PML85_13895) 28585..28680 + 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
PML85_RS13900 (PML85_13900) 28844..29581 + 738 WP_010725603.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PML85_RS13905 (PML85_13905) 29751..30011 + 261 Protein_31 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
PML85_RS13910 (PML85_13910) 30114..31010 + 897 WP_272019756.1 ParA family protein -
PML85_RS13915 (PML85_13915) 31081..31296 + 216 WP_002295735.1 peptide-binding protein -
PML85_RS13920 (PML85_13920) 31313..31585 + 273 WP_000301765.1 antitoxin Antitoxin
PML85_RS13925 (PML85_13925) 31587..32450 + 864 WP_010717401.1 zeta toxin family protein Toxin
PML85_RS13930 (PML85_13930) 32891..33208 + 318 WP_002300567.1 hypothetical protein -
PML85_RS13935 (PML85_13935) 33741..34238 + 498 WP_115253289.1 molecular chaperone DnaJ -
PML85_RS13940 (PML85_13940) 34273..34596 + 324 WP_047342412.1 DpnD/PcfM family protein -
PML85_RS13945 (PML85_13945) 34625..34882 - 258 WP_202243043.1 hypothetical protein -
PML85_RS13950 (PML85_13950) 34885..35184 - 300 WP_002325624.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) - 1..41241 41241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32521.11 Da        Isoelectric Point: 6.9965

>T268847 WP_010717401.1 NZ_CP116551:31587-32450 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEVIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLRPPTPPIPKTPKLPGL

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT268847 WP_000301765.1 NZ_CP116551:31313-31585 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References