Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 508945..509516 Replicon chromosome
Accession NZ_CP116549
Organism Enterococcus faecium strain K80-15b

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag PML85_RS02435 Protein ID WP_002286801.1
Coordinates 509175..509516 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag PML85_RS02430 Protein ID WP_002323011.1
Coordinates 508945..509175 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML85_RS02405 (PML85_02405) 504078..505409 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
PML85_RS02410 (PML85_02410) 505431..506057 + 627 WP_002286816.1 cysteine hydrolase -
PML85_RS02415 (PML85_02415) 506240..506821 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
PML85_RS02420 (PML85_02420) 507553..508115 + 563 Protein_480 SOS response-associated peptidase family protein -
PML85_RS02425 (PML85_02425) 508320..508658 - 339 WP_002286804.1 hypothetical protein -
PML85_RS02430 (PML85_02430) 508945..509175 + 231 WP_002323011.1 hypothetical protein Antitoxin
PML85_RS02435 (PML85_02435) 509175..509516 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PML85_RS02440 (PML85_02440) 510366..510551 + 186 WP_002304207.1 hypothetical protein -
PML85_RS02445 (PML85_02445) 511056..511250 + 195 WP_002297028.1 hypothetical protein -
PML85_RS02450 (PML85_02450) 511441..511690 + 250 Protein_486 transposase -
PML85_RS02455 (PML85_02455) 511934..512764 - 831 WP_002286789.1 manganese catalase family protein -
PML85_RS02460 (PML85_02460) 512972..514306 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T268845 WP_002286801.1 NZ_CP116549:509175-509516 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT268845 WP_002323011.1 NZ_CP116549:508945-509175 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References