268840

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 73057..74165 Replicon plasmid pK70-1a-A
Accession NZ_CP116544
Organism Enterococcus faecium strain K70-1a

Toxin (Protein)


Gene name MNTss Uniprot ID A0A3S8RBH6
Locus tag PML99_RS13675 Protein ID WP_071661597.1
Coordinates 73057..73926 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag PML99_RS13680 Protein ID WP_000205227.1
Coordinates 73941..74165 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML99_RS13645 (PML99_13645) 68842..69384 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
PML99_RS13650 (PML99_13650) 69381..70178 - 798 Protein_81 aminoglycoside 6-adenylyltransferase -
PML99_RS13655 (PML99_13655) 70348..71787 - 1440 WP_001028144.1 aminoglycoside O-phosphotransferase APH(2'')-Ia -
PML99_RS13660 (PML99_13660) 71788..72180 - 393 WP_000393259.1 GNAT family N-acetyltransferase -
PML99_RS13665 (PML99_13665) 72199..72309 - 111 Protein_84 aminoglycoside 6-adenylyltransferase -
PML99_RS13670 (PML99_13670) 72342..73076 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
PML99_RS13675 (PML99_13675) 73057..73926 - 870 WP_071661597.1 nucleotidyltransferase domain-containing protein Toxin
PML99_RS13680 (PML99_13680) 73941..74165 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
PML99_RS13685 (PML99_13685) 74583..75884 + 1302 Protein_88 ISL3 family transposase -
PML99_RS13690 (PML99_13690) 76193..76996 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
PML99_RS13695 (PML99_13695) 77050..78534 - 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32902.55 Da        Isoelectric Point: 4.9770

>T268840 WP_071661597.1 NZ_CP116544:c73926-73057 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT268840 WP_000205227.1 NZ_CP116544:c74165-73941 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S8RBH6

Antitoxin

Source ID Structure

References