Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 516850..517421 Replicon chromosome
Accession NZ_CP116536
Organism Enterococcus faecium strain K162-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A8B2RQB5
Locus tag PML70_RS02470 Protein ID WP_002349927.1
Coordinates 517080..517421 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A854KBC5
Locus tag PML70_RS02465 Protein ID WP_010728130.1
Coordinates 516850..517080 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML70_RS02440 (PML70_02440) 512074..513405 + 1332 WP_002300334.1 FAD-containing oxidoreductase -
PML70_RS02445 (PML70_02445) 513427..514053 + 627 WP_002300333.1 cysteine hydrolase -
PML70_RS02450 (PML70_02450) 514246..514827 + 582 WP_002300332.1 TetR/AcrR family transcriptional regulator -
PML70_RS02455 (PML70_02455) 515436..516011 + 576 WP_002349929.1 SOS response-associated peptidase family protein -
PML70_RS02460 (PML70_02460) 516216..516554 - 339 WP_002286804.1 hypothetical protein -
PML70_RS02465 (PML70_02465) 516850..517080 + 231 WP_010728130.1 hypothetical protein Antitoxin
PML70_RS02470 (PML70_02470) 517080..517421 + 342 WP_002349927.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PML70_RS02475 (PML70_02475) 518270..518455 + 186 WP_002304207.1 hypothetical protein -
PML70_RS02480 (PML70_02480) 518960..519154 + 195 WP_002295146.1 hypothetical protein -
PML70_RS02485 (PML70_02485) 519345..519594 + 250 Protein_493 transposase -
PML70_RS02490 (PML70_02490) 519838..520668 - 831 WP_002286789.1 manganese catalase family protein -
PML70_RS02495 (PML70_02495) 520876..522210 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13216.59 Da        Isoelectric Point: 10.0749

>T268832 WP_002349927.1 NZ_CP116536:517080-517421 [Enterococcus faecium]
VSGERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8522.45 Da        Isoelectric Point: 4.0537

>AT268832 WP_010728130.1 NZ_CP116536:516850-517080 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIKDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2RQB5

Antitoxin

Source ID Structure
AlphaFold DB A0A854KBC5

References