Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 479517..480088 Replicon chromosome
Accession NZ_CP116532
Organism Enterococcus faecium strain K191-3

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag PML76_RS02290 Protein ID WP_002286801.1
Coordinates 479747..480088 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag PML76_RS02285 Protein ID WP_002323011.1
Coordinates 479517..479747 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML76_RS02260 (PML76_02260) 474884..476215 + 1332 WP_002330022.1 FAD-containing oxidoreductase -
PML76_RS02265 (PML76_02265) 476237..476863 + 627 WP_002286816.1 cysteine hydrolase -
PML76_RS02270 (PML76_02270) 477046..477627 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
PML76_RS02275 (PML76_02275) 478112..478687 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
PML76_RS02280 (PML76_02280) 478892..479230 - 339 WP_002286804.1 hypothetical protein -
PML76_RS02285 (PML76_02285) 479517..479747 + 231 WP_002323011.1 hypothetical protein Antitoxin
PML76_RS02290 (PML76_02290) 479747..480088 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PML76_RS02295 (PML76_02295) 480938..481123 + 186 WP_002304207.1 hypothetical protein -
PML76_RS02300 (PML76_02300) 481393..482555 + 1163 WP_086956687.1 IS3 family transposase -
PML76_RS02305 (PML76_02305) 482686..482928 + 243 Protein_457 LPXTG cell wall anchor domain-containing protein -
PML76_RS02310 (PML76_02310) 483002..483895 + 894 WP_002286772.1 class C sortase -
PML76_RS02315 (PML76_02315) 484079..484753 + 675 WP_002286771.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T268830 WP_002286801.1 NZ_CP116532:479747-480088 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT268830 WP_002323011.1 NZ_CP116532:479517-479747 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References