268828

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 479513..480084 Replicon chromosome
Accession NZ_CP116528
Organism Enterococcus faecium strain K188-5

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag PML77_RS02280 Protein ID WP_002286801.1
Coordinates 479743..480084 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag PML77_RS02275 Protein ID WP_002323011.1
Coordinates 479513..479743 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML77_RS02250 (PML77_02250) 474880..476211 + 1332 WP_002330022.1 FAD-containing oxidoreductase -
PML77_RS02255 (PML77_02255) 476233..476859 + 627 WP_002286816.1 cysteine hydrolase -
PML77_RS02260 (PML77_02260) 477042..477623 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
PML77_RS02265 (PML77_02265) 478108..478683 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
PML77_RS02270 (PML77_02270) 478888..479226 - 339 WP_002286804.1 hypothetical protein -
PML77_RS02275 (PML77_02275) 479513..479743 + 231 WP_002323011.1 hypothetical protein Antitoxin
PML77_RS02280 (PML77_02280) 479743..480084 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PML77_RS02285 (PML77_02285) 480934..481119 + 186 WP_002304207.1 hypothetical protein -
PML77_RS02290 (PML77_02290) 481389..482551 + 1163 WP_086956687.1 IS3 family transposase -
PML77_RS02295 (PML77_02295) 482682..482924 + 243 Protein_455 LPXTG cell wall anchor domain-containing protein -
PML77_RS02300 (PML77_02300) 482998..483891 + 894 WP_002286772.1 class C sortase -
PML77_RS02305 (PML77_02305) 484075..484749 + 675 WP_002286771.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T268828 WP_002286801.1 NZ_CP116528:479743-480084 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT268828 WP_002323011.1 NZ_CP116528:479513-479743 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References