Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 492022..492593 Replicon chromosome
Accession NZ_CP116514
Organism Enterococcus faecium strain K195-6b

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag PML82_RS02360 Protein ID WP_131773576.1
Coordinates 492252..492593 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag PML82_RS02355 Protein ID WP_002323011.1
Coordinates 492022..492252 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML82_RS02330 (PML82_02330) 487377..488708 + 1332 WP_002300334.1 FAD-containing oxidoreductase -
PML82_RS02335 (PML82_02335) 488730..489356 + 627 WP_002300333.1 cysteine hydrolase -
PML82_RS02340 (PML82_02340) 489549..490130 + 582 WP_002300332.1 TetR/AcrR family transcriptional regulator -
PML82_RS02345 (PML82_02345) 490616..491191 + 576 WP_002302305.1 SOS response-associated peptidase family protein -
PML82_RS02350 (PML82_02350) 491396..491734 - 339 WP_002286804.1 hypothetical protein -
PML82_RS02355 (PML82_02355) 492022..492252 + 231 WP_002323011.1 hypothetical protein Antitoxin
PML82_RS02360 (PML82_02360) 492252..492593 + 342 WP_131773576.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PML82_RS02365 (PML82_02365) 493443..493628 + 186 WP_002325132.1 hypothetical protein -
PML82_RS02370 (PML82_02370) 494133..494312 + 180 WP_131773577.1 hypothetical protein -
PML82_RS02375 (PML82_02375) 494316..495275 - 960 WP_002301399.1 IS30 family transposase -
PML82_RS02380 (PML82_02380) 495396..497093 + 1698 WP_002326336.1 SpaH/EbpB family LPXTG-anchored major pilin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 494316..495275 959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13287.67 Da        Isoelectric Point: 10.0749

>T268818 WP_131773576.1 NZ_CP116514:492252-492593 [Enterococcus faecium]
VSEERIYIPKKGNIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT268818 WP_002323011.1 NZ_CP116514:492022-492252 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References