268816

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 137..18136 Replicon plasmid pK205-4a-B
Accession NZ_CP116512
Organism Enterococcus gallinarum strain K205-4a

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag PML87_RS15145 Protein ID WP_001284311.1
Coordinates 139..1002 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag PML87_RS15140 Protein ID WP_000301765.1
Coordinates 18136..137 (+) Length -5999.3333333333 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML87_RS15145 (PML87_15145) 139..1002 + 864 WP_001284311.1 zeta toxin family protein Toxin
PML87_RS15150 (PML87_15150) 1265..2002 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PML87_RS15155 (PML87_15155) 2127..2210 - 84 WP_033602770.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
PML87_RS15160 (PML87_15160) 2769..3857 - 1089 WP_231415007.1 MobV family relaxase -
PML87_RS15165 (PML87_15165) 4341..4484 + 144 WP_002360221.1 hypothetical protein -
PML87_RS15170 (PML87_15170) 4738..6165 + 1428 WP_131648054.1 chloramphenicol/florfenicol efflux MFS transporter FexA -
PML87_RS15175 (PML87_15175) 6343..6759 + 417 WP_000855243.1 NAD(P)H-dependent oxidoreductase -
PML87_RS15180 (PML87_15180) 6901..7311 + 411 WP_218210702.1 hypothetical protein -
PML87_RS15185 (PML87_15185) 7470..9383 + 1914 WP_272019623.1 ABC-F type ribosomal protection protein OptrA -
PML87_RS15190 (PML87_15190) 9639..11219 + 1581 WP_125276230.1 IS21 family transposase -
PML87_RS15195 (PML87_15195) 11374..11646 + 273 WP_011266100.1 helix-turn-helix transcriptional regulator -
PML87_RS15200 (PML87_15200) 12030..13520 + 1491 WP_001025003.1 primase C-terminal domain-containing protein -
PML87_RS15205 (PML87_15205) 13868..14038 + 171 WP_000713594.1 hypothetical protein -
PML87_RS15210 (PML87_15210) 14052..14669 + 618 WP_001062589.1 recombinase family protein -
PML87_RS15215 (PML87_15215) 14669..16813 + 2145 WP_000108741.1 type IA DNA topoisomerase -
PML87_RS15220 (PML87_15220) 16916..17812 + 897 WP_001835294.1 ParA family protein -
PML87_RS15225 (PML87_15225) 17904..18119 + 216 WP_001835296.1 peptide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / fexA / optrA - 1..18271 18271


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T268816 WP_001284311.1 NZ_CP116512:139-1002 [Enterococcus gallinarum]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: -5999.3333333333 a.a.        Molecular weight: Da        Isoelectric Point:

>AT268816 WP_000301765.1 NZ_CP116512:18136-137 [Enterococcus gallinarum]

Download         Length: -17998 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References