Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5443706..5444331 Replicon chromosome
Accession NZ_CP116491
Organism Klebsiella pneumoniae strain XY05-03

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PIB23_RS27070 Protein ID WP_002882817.1
Coordinates 5443706..5444089 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PIB23_RS27075 Protein ID WP_004150355.1
Coordinates 5444089..5444331 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB23_RS27055 (PIB23_27055) 5441072..5441974 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PIB23_RS27060 (PIB23_27060) 5441971..5442606 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PIB23_RS27065 (PIB23_27065) 5442603..5443532 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PIB23_RS27070 (PIB23_27070) 5443706..5444089 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PIB23_RS27075 (PIB23_27075) 5444089..5444331 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PIB23_RS27080 (PIB23_27080) 5444536..5445453 + 918 WP_002882812.1 alpha/beta hydrolase -
PIB23_RS27085 (PIB23_27085) 5445467..5446408 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PIB23_RS27090 (PIB23_27090) 5446453..5446890 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PIB23_RS27095 (PIB23_27095) 5446887..5447747 - 861 WP_002882807.1 virulence factor BrkB family protein -
PIB23_RS27100 (PIB23_27100) 5447741..5448340 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T268801 WP_002882817.1 NZ_CP116491:c5444089-5443706 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT268801 WP_004150355.1 NZ_CP116491:c5444331-5444089 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References