Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 107084..107727 Replicon plasmid unnamed1
Accession NZ_CP116481
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag E5Q65_RS23930 Protein ID WP_001044768.1
Coordinates 107311..107727 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag E5Q65_RS23925 Protein ID WP_001261287.1
Coordinates 107084..107314 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS23915 (102262) 102262..103395 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
E5Q65_RS23920 (103658) 103658..106777 - 3120 WP_023909028.1 hypothetical protein -
E5Q65_RS23925 (107084) 107084..107314 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
E5Q65_RS23930 (107311) 107311..107727 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
E5Q65_RS23935 (107889) 107889..108896 - 1008 Protein_131 AAA family ATPase -
E5Q65_RS23940 (108959) 108959..109663 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
E5Q65_RS23945 (109716) 109716..109940 + 225 Protein_133 DDE-type integrase/transposase/recombinase -
E5Q65_RS23950 (110126) 110126..111109 + 984 WP_113962661.1 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / catA1 / sul1 / qacE / aadA2 / dfrA12 / qepA4 / mph(A) / erm(B) / msr(E) / mph(E) / qnrB4 / blaDHA-1 - 1..138420 138420


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T268766 WP_001044768.1 NZ_CP116481:107311-107727 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT268766 WP_001261287.1 NZ_CP116481:107084-107314 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References