Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3771317..3771893 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag E5Q65_RS18240 Protein ID WP_001350372.1
Coordinates 3771317..3771604 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag E5Q65_RS18245 Protein ID WP_001402821.1
Coordinates 3771591..3771893 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS18225 (3766899) 3766899..3768281 - 1383 WP_023155808.1 restriction endonuclease subunit S -
E5Q65_RS18230 (3768271) 3768271..3769890 - 1620 WP_001029742.1 class I SAM-dependent DNA methyltransferase -
E5Q65_RS18235 (3769954) 3769954..3771120 - 1167 WP_001672354.1 restriction endonuclease -
E5Q65_RS18240 (3771317) 3771317..3771604 + 288 WP_001350372.1 BrnT family toxin Toxin
E5Q65_RS18245 (3771591) 3771591..3771893 + 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
E5Q65_RS18250 (3771927) 3771927..3772883 - 957 WP_001295525.1 GTPase -
E5Q65_RS18255 (3772894) 3772894..3773097 - 204 WP_000467859.1 YbdD/YjiX family protein -
E5Q65_RS18260 (3773147) 3773147..3775297 - 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
E5Q65_RS18265 (3775690) 3775690..3776202 - 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T268757 WP_001350372.1 NZ_CP116480:3771317-3771604 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT268757 WP_001402821.1 NZ_CP116480:3771591-3771893 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References