Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3537139..3537636 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag E5Q65_RS17050 Protein ID WP_001173343.1
Coordinates 3537463..3537636 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag E5Q65_RS17045 Protein ID WP_000398619.1
Coordinates 3537139..3537435 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS17025 (3533870) 3533870..3534589 - 720 WP_000611288.1 two-component system response regulator DcuR -
E5Q65_RS17030 (3534586) 3534586..3536217 - 1632 WP_001216460.1 sensor histidine kinase -
E5Q65_RS17035 (3536398) 3536398..3536628 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
E5Q65_RS17040 (3536640) 3536640..3536912 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
E5Q65_RS17045 (3537139) 3537139..3537435 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
E5Q65_RS17050 (3537463) 3537463..3537636 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
E5Q65_RS17055 (3537755) 3537755..3539272 - 1518 WP_023155344.1 lysine--tRNA ligase -
E5Q65_RS17060 (3539509) 3539509..3540966 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268755 WP_001173343.1 NZ_CP116480:3537463-3537636 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268755 WP_000398619.1 NZ_CP116480:3537139-3537435 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References