Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2797953..2798539 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag E5Q65_RS13640 Protein ID WP_042014162.1
Coordinates 2798309..2798539 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E5Q65_RS13635 Protein ID WP_000593555.1
Coordinates 2797953..2798312 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS13610 (2794085) 2794085..2795029 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
E5Q65_RS13615 (2795026) 2795026..2795859 + 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
E5Q65_RS13620 (2795859) 2795859..2796623 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
E5Q65_RS13625 (2796620) 2796620..2797426 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
E5Q65_RS13630 (2797432) 2797432..2797833 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
E5Q65_RS13635 (2797953) 2797953..2798312 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E5Q65_RS13640 (2798309) 2798309..2798539 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
E5Q65_RS13645 (2798643) 2798643..2799767 - 1125 WP_001314210.1 ABC transporter permease -
E5Q65_RS13650 (2799767) 2799767..2802502 - 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T268753 WP_042014162.1 NZ_CP116480:c2798539-2798309 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT268753 WP_000593555.1 NZ_CP116480:c2798312-2797953 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References