Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2204032..2204686 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag E5Q65_RS10625 Protein ID WP_000244772.1
Coordinates 2204032..2204439 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag E5Q65_RS10630 Protein ID WP_000354046.1
Coordinates 2204420..2204686 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS10605 (2199989) 2199989..2201722 - 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -
E5Q65_RS10610 (2201728) 2201728..2202438 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
E5Q65_RS10615 (2202463) 2202463..2203359 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
E5Q65_RS10620 (2203471) 2203471..2203992 + 522 WP_001055874.1 flavodoxin FldB -
E5Q65_RS10625 (2204032) 2204032..2204439 - 408 WP_000244772.1 protein YgfX Toxin
E5Q65_RS10630 (2204420) 2204420..2204686 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
E5Q65_RS10635 (2204929) 2204929..2205909 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
E5Q65_RS10640 (2205986) 2205986..2206645 - 660 WP_000250274.1 hemolysin III family protein -
E5Q65_RS10645 (2206809) 2206809..2207120 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
E5Q65_RS10650 (2207165) 2207165..2208598 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
E5Q65_RS10655 (2208655) 2208655..2209398 - 744 WP_023155906.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T268751 WP_000244772.1 NZ_CP116480:c2204439-2204032 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT268751 WP_000354046.1 NZ_CP116480:c2204686-2204420 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References