Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1730585..1731210 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag E5Q65_RS08435 Protein ID WP_000911329.1
Coordinates 1730585..1730983 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag E5Q65_RS08440 Protein ID WP_000450524.1
Coordinates 1730983..1731210 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS08415 (1726463) 1726463..1726663 + 201 WP_000383836.1 YpfN family protein -
E5Q65_RS08420 (1726773) 1726773..1727471 - 699 WP_000679823.1 esterase -
E5Q65_RS08425 (1727545) 1727545..1729560 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
E5Q65_RS08430 (1729575) 1729575..1730438 - 864 WP_001267498.1 neutral zinc metallopeptidase -
E5Q65_RS08435 (1730585) 1730585..1730983 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
E5Q65_RS08440 (1730983) 1730983..1731210 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
E5Q65_RS08445 (1731364) 1731364..1732077 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
E5Q65_RS08450 (1732290) 1732290..1733324 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
E5Q65_RS08455 (1733341) 1733341..1734219 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
E5Q65_RS08460 (1734365) 1734365..1734937 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
E5Q65_RS08465 (1734937) 1734937..1735407 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T268749 WP_000911329.1 NZ_CP116480:c1730983-1730585 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT268749 WP_000450524.1 NZ_CP116480:c1731210-1730983 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References