Detailed information of TA system TA026874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3163979..3164666
• Replicon: chromosome
• Accession: NC_017522
• Organism: Mycobacterium tuberculosis CCDC5180

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: CCDC5180_RS15145
• Protein ID: WP_003414624.1
• Coordinates: 3164223..3164666 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: CCDC5180_RS15140
• Protein ID: WP_003414620.1
• Coordinates: 3163979..3164236 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCDC5180_RS15120	3159299..3160153	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
CCDC5180_RS15125	3160209..3161372	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
CCDC5180_RS15130	3161389..3162603	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
CCDC5180_RS15135	3162611..3163852	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
CCDC5180_RS15140	3163979..3164236	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
CCDC5180_RS15145	3164223..3164666	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCDC5180_RS15150	3164746..3165408	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
CCDC5180_RS15155	3165504..3165695	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CCDC5180_RS15160	3166027..3167775	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
CCDC5180_RS15165	3167871..3168452	+	582	WP_003414644.1	fasciclin domain-containing protein	-
CCDC5180_RS15170	3168552..3168818	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T26874 WP_003414624.1 NC_017522:3164223-3164666 [Mycobacterium tuberculosis CCDC5180]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T26874 NC_017522:3164223-3164666 [Mycobacterium tuberculosis CCDC5180]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT26874 WP_003414620.1 NC_017522:3163979-3164236 [Mycobacterium tuberculosis CCDC5180]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT26874 NC_017522:3163979-3164236 [Mycobacterium tuberculosis CCDC5180]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0